Mouse Lymphoma Assay

The mouse lymphoma assay (mutagenicity assay in vitro: mammalian cell gene mutation test) detects mutagenic and clastogenic effects of the test item in mammalian cells. The assay is performed according to the OECD guideline 476. Screening and ranking study designs are also available.

Quantity of test item required: 1 g
Turnaround time of draft report : 20 working days


Study Design

• cytotoxicity and range finding pre-test
• L5178Y/TK^+/- mouse lymphoma cells, heterozygous at the thymidine kinase (TK) locus
• two replicate cultures per dose level
• three subcultures per replicate
• treatment: 3 h w and w/o metabolic activation, 24 h w/o metabolic activation
• four dose levels
• negative (vehicle) control
• positive control
• plating efficiency control
• selection period: 10 - 14 days

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