Micronucleus Test

The micronucleus test in vitro detects clastogenic effects of the test item in mammalian cells. The test is performed according to the OECD draft guideline by J. M. Parry. Screening and ranking study designs are also available.

Standard Micronucleus Test (OECD Draft Guideline by J. M. Parry)

Quantity of test item required: 2 g
Turnaround time of draft report : 20 working days


Study Design

• cytotoxicity and range finding pre-test
• V79 cells
• duplicates
• three dose levels
• with and without rat S9
• incubation time: 3 h w and w/o S9, 24 h w/o S9
• negative control
• positive control
• microscopic analysis: 1000 cells per duplicate

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