HPRT Test

The H(G)PRT test (hypoxanthine guanine phosphoribosyl transferase test; mutagenicity assay in vitro: mammalian cell gene mutation test) detects mutagenic effects of the test item in mammalian cells. The test is performed according to OECD guideline 476. Screening and ranking study designs are also available.

Standard HPRT Test (OECD 476)

Quantity of test item required: 3 g
Turnaround time of draft report : 35 working days

Study Design

• cytotoxicity and range finding pre-test
• V79 cells
• four dose levels
• two replicate culture per dose level
• three subcultures per replicate
• treatment: 4 h or 24 h w/o metabolic activation, 4 h with metabolic activation
• plating efficiency control
• negative control
• positive control

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