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HPRT Test
The H(G)PRT test (hypoxanthine guanine phosphoribosyl transferase test; mutagenicity assay in vitro: mammalian cell gene mutation test) detects mutagenic effects of the test item in mammalian cells. The test is performed according to OECD guideline 476. Screening and ranking study designs are also available.
Advanced HPRT Test (V79 Cell BatteryTM, OECD 47)
Quantity of test item required: 2 g
Turnaround time of draft report : 35 working days
Study Design
- cytotoxicity and range finding pre-test
- recombinant V79 cells expressing phase I and / or phase II enzymes (see V79 Cell BatteryTM)
- internal metabolic activation
- four dose levels
- two replicate cultures per dose level
- three subcultures per replicate
- treatment: 24 to 72 h
- plating efficiency control
- negative control
- positive control
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