Comet Assay

The comet assay (single-cell gel elektrophoresis assay, SCGE) measures DNA strand breaks at the level of single cell from any tissue or cell culture.
The assay is performed according to "Guidelines for in vitro and in vivo Genetic Toxicology Testing: Single Cell Gel / Comet Assay" by R. Tice et al. (2000). Screening and ranking study designs are also available.

Standard Comet Assay (Guideline by R. Tice et al., 2000)

Quantity of test item required: 500 g
Turnaround time of draft report : 15 working days

Study Design

• cytotoxicity and range finding pre-test
• V79 cells or others
• duplicates
• five dose levels
• treatment: 3 - 6 h w and w/o metabolic activation, 24 h w/o metabolic activation
• negative (vehicle) control
• positive control
• microscopic analysis: 100 cells per duplicate

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