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Comet Assay
The comet assay (single-cell gel elektrophoresis assay, SCGE) measures DNA strand breaks at the level of single cells from any tissue or cell culture.
The assay is performed according to "Guidelines for in vitro and in vivo Genetic Toxicology Testing: Single Cell Gel / Comet Assay" by R. Tice et al. (2000). Screening and ranking study designs are also available.
Advanced Comet Assay (V79 Cell BatteryTM, Guideline by R. Tice et al., 2000)
Quantity of test item required: 500 mg
Turnaround time of draft report : 5 working days
Study Design
- recombinant V79 cells expressing phase I and / or phase II enzymes (see V79 Cell BatteryTM)
- internal metabolic activation
- duplicates
- five dose levels
- treatment: 24 - 72 h
- negative (vehicle) control
- positive control
- microscopic analysis: 100 cells per duplicate
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